김진경

인사말

김진경 교수

전문분야 | 분자약학 및 저분자 생물학
전화번호 | 031-881-7018
E – mail | kyeoung@cha.ac.kr

​학위

 

대구대학교 생물공학과 ( 공학사 )
일본 히로시마대학교 종합약학과 ( 약학석사 )
일본 히로시마대학교 종합약학과 ( 약학박사 )

주요경력

 

충남대학교 암공동연구소 박사후연구원
일본 과학기술진흥사업단 (ERATO) 연구원
포천중문의과대학교 의학부 조교수 (생화학교실)
포천중문의과대학교 생명과학전문대학원 부교수
포천중문의과대학교 생명과학전문대학원 교학부장
차 의과학대학교 약학과 학과장
(현) 차 의과학대학교 약학대학 교수 (생화학전공)
(현) 차 의과학대학교 교무처장

일본 히로시마대학교 의학부 연구원
일본 히로시마대학교 종합약학과 Teaching Assistant
일본 정부 문부성 국가장학생
한국동물학회 뉴스지 편집위원
한국생화학분자생물학회 뉴스지 편집위원
중소기업기술개발 지원사업 평가단 위원
(현) 지식경제기술혁신 평가단 위원
(현) 중앙약사심의위원회 전문가
(현) 한국약학회 약학회지 편집위원
(현) 한국약학교육협의회 총무재정위원회 위원

 

주요 연구내용

유전자조실연구실은, 사람 세포를 이용하여 노화, 각종 암, 줄기세포에서 특이적으로 발현하는 신규 mRNA or miRNA를 cloning하여 세포 내에서의 기능을 연구 하고 있으며, 세부적으로는
1)  hMSC의 대량 배양을 위한 hMSC에 존재하는 신규 mRNA/miRNA의 확인 및 기능 연구
2)  각종 암세포에 존재하는 신규 mRNA/miRNA의 확인 및 기능 연구
3)  각종 노화세포에서 특이적으로 발현하는 신규 miRNA(가칭 107)의 기능 연구
4)  유전자 진단 kit 개발을 위한 암세포에서 특이적인 신규 유전자 (imup-1,2)의 기능 연구

 

연구실 소개

MicroRNA(miRNA)는 대략 22nt 정도로 단백질을 암호화하지 않는 small RNA이다. 이들은 표적 유잔자의  전사 후 과정에 작용하여 단백질 번역 수준을 조절하는 것으로 보고되고 있다. miRNA의 생물학적 기능은 세포의 발달, 분화, 증식, 세포사멸, 스트레스반응, 지방대사, 인슐린 분비, 발암과정 등의 광범위한 biological process에 관여한다고 보고되고 있으며, 새로운 조절 물질로 각광받고 있다.

본 연구실은,  인간 세포를 이용하여 노화, 각종 암, 줄기세포에 특이적으로 발현하는 신규mRNA or miRNA를 cloning하여 세포 내에서의 기능을 연구하는 것을 목적으로 한다. 현재 22종의 신규 유전자와 17종의 신규 miRNA를 동정하여 각종 세포에서 특이적인 mRNA/miRNA의 database를 구축하였다. 따라서 본 연구를 통하여 각 miRNA의 고유한 기능을 밝혀내는 것은 뉴 밀레니엄 의료 바이오 산업의 성장 동력자산으로서 뿐 아니라 범 세계적 생명약학을 선도할 것으로 기대한다.

[발표논문]

 

국내논문 (* correspondence)

 

JJ Lee, JK Yoo, JK Kim, SW Son*. Expression and Purification of Unstructured Protein, IMUP-1, using Chaperone Co-expression System for NMR Study. 한국자기공명학회. 17(1):30-39. 2013

HN Yoo, JK Yoo, SJ Choi, JK Kim*. Expression of C6orf62 in human embryonic stem cells and cancer cells. Reprod Dev Biol. 34(3):229-233. 2010

전수연, 이현정, 정현민, 김진경, 김기진*. 태반내 Immortalization-upregulated Proteins-2[IMUP-2] 발현. 대한생식의학회지. 36(3):163-173. 2009

KS Shin, KH Na, HJ Lee, DG Kim, SJ Shin, JK Kim, GJ Kim*. Characterization of Fetal Tissue-derived Mesenchymal Stem Cells. Int J Stem Cells. 2(1):51-58. 2009

JK Yoo, DR Lee, JJ Lim, JK Kim*. Expression of HBP2 in Human Spermatogonial Stem Cell-like Cells from Nonobstructive Azoospermia Patients and Its Role in G1/S Transition & Downregulation in Colon Cancer. Reprod Dev Biol. 32(4):211-215. 2008

JS Ahn, SH Moon, JK Yoo, HM Jung, HM Chung, JK Kim*. Expression of the C1orf31 gene in Human Stem Cells and Cancer Cells. Reprod Dev Biol. 32(4):223-227. 2008

HM Jung, SJ Choi, SH Kim, SH Moon, JK Yoo, HM Chung, JK Kim*. Expression of HERV-HX2 in Cancer Cells and Human Embryonic Stem Cells. Reprod Dev Biol. 32(2)105-110. 2008

HM Noh, SJ Choi, SH Kim, KS Kim, JK Kim*. Differential Expression of TPX2 upon Differentiation of Human Embryonic Stem Cells. Reprod Dev Biol. 31(4):221-226. 2007

HJ Choi, GI Kim, HJ Kim, JH Min, HM Noh, KS Kim, JK Kim*. Discovery of Differentially Overexpressed Genes in Immortalized Cells and Human Pulmonary Non-small Cell Carcinomas. J Lung Cancer. C5(2):96-101. 2006

JW Kim, DY Oh, SY Chong, DJ Yim, JK Kim, NK Kim*. Distributions of MTHFR Gene Polymorphism according to the Location of Colon Cancer. J Korean Soc Coloproctol. 22(2):69-74. 2006

JY Ahn, NM Yu, IB Han, JK Kim*. Characterization of the Differentially Expressed Novel Genes in Human Glioblastoma using Subtractive Hybridization. 대한뇌종양학회지. 4(2):1-5. 2005

EJ Choi, HJ An, NM Yu, JK Kim*. Characterization of the Differentially Expressed Genes in Human Breast Cancer using Subtractive Hybridization. 한국유방암학회지. 8(2):10-16. 2005

NM Yu, JY Ahn, EJ Choi, YK Hong, TG Kim, CH Kim, KS Lee, DS Kim, JK Kim*. Detection of Differentially Expressed Genes in Glioblastoma by Suppression Subtractive Hybridization. 대한신경외과학회지. 37(6):443-448. 2005

김계성, 김진경*. 배아줄기세포를 이용한 포유류 초기 신경계 발달의 이해. 생화학분자생물학뉴스. 12:261-267. 2004

NM Yu, JY Ahn, KS Kim, CH Kim, TG Kim, EJ Choi, JK Kim*. Isolation and Characterization of Differentially Expressed Genes in Human Glioblastoma using Suppression Subtractive Hybridization. 대한뇌종양학회지 3(2):88-94. 2004

JY Ahn, JK Kim, YS Chung, SS Chung, JY Joo*. Molecular Genetic of Primary Intracerebral Hemorrhage. 대한뇌혈관외과학회지. 6(1):5-10. 2004

김선희, 김남근, 조성훈, 한진희, 임동진, 곽선영, 조윤경, 김진경, 이수만, 황성규, 오도연*. 한국인 1,647명에서 Thymidylate Synthase Enhancer Region (TSER)의 유전적 다형 연구 (Genetic Polymorphisms of Thymidylate Synthase Enhancer Region (TSER) in 1,674 Koreans). 한국혈전지혈학회지. 10:11-20. 2003

JK Kim, DY Oh, SY K, JH Han, YS Chung, NK Kim*. Genetic Polymorphism of Vascular Endothelial Growth Factor (VEGF C936T) in Koreans. Korean J Biol Sci. 7(3):216-264. 2003

SC Yoon, SH Ahn, YJ Mun, JK Kim, YK Choo, KY Jung, YM Kim, WH Woo*. Studies on the Antitumor Activity of Gamisoam-san via Suppressing Angiogenesis and Growth Factor Expression. 동의생리병리학회지. 17(4):969-979. 2003

JY Ahn, JK Kim*. Molecular Genetics of Cerebral Aneurysm. 대한뇌혈관외과학회지. 5(1):12-16. 2003

JY Ahn, NK Kim, JK Kim, JY Joo, KS Lee, JU Cho*. Expression of the DNA repair gene, N-methylpurine-DNA glycosylase in astrocytic tumors. 대한신경외과학회지. 33(3):241-246. 2003

윤성찬, 안성훈, 문연자, 김진경, 추영국, 정규용, 우원홍*. Studies on the antimetastasis & antiangiogenesis effects of Gamisoamsan. 대한한의학방제학회지 10(2):113-126. 2002

JY Ahn, NK Kim, JH Han, JK Kim, JY Joo, KS Lee*. Genetic Mutation of 5,10-Methylenetetrahydrofolate Reductase in the Brain Neoplasm. 대한신경외과학회지 32(3):183-188. 2002

JK Kim*. Identification of Bak-like protein cDNA. Yakhak Hoeji. 45(4):426-430. 2001

JK Kim*. Aging and lifespan of human somatic cells. 한국동물학회지(뉴스레터). 18(1):10-15. 2001

김진경*. 게놈(Genome), 그 빛과 그림자. 차병원보. 76:14-15. 2001

김삼용, 윤소현, 김현수, 김종옥, 윤환중, 김진경, 조덕연*. 항암제 처리한 백혈병 세포주에서의 Apoptosis 발현. 충남의대잡지. 25(1):177-183

 

국외논문 (* correspondence)

 

JK Yoo, CH Kim, HY Jung, DR Lee, JK Kim*. Discovery and characterization of miRNA during cellular senescence in bone marrow-derived human mesenchymal stem cells. 58:139-145. 2014

HB Lee, Y Kim, H Yoo, JM Lee, YK Kim, NK Kim, JK Kim, SH oh. Blood genomic profiling in extracranial- and intracranial atherosclerosis in ischemic strock patients. 134:686-692. 2014

HJ Lee, JH Choi, Jung J, JK kim, SS Lee, GJ Kim. Changes in PTTG1 by human TERT gene expression modulate the self-renewal of placent-derived mesenchymal stem cells. Cell Tissue Res. 357:145-157. 2014

JK Yoo, HY Jung, JM Lee, HS Yi, SH Oh, HY Ki, H Yoo, HR Kim, HS song, SH Kim*, JK Kim*. The novel miR-9500 regulates the proliferation and migration of human lung cancer cells by targeting Akt1. Cell Death Differ. 21:1150-11599. 2014

MK Park, HJ Lee, JK Choi, HJ Kim, JH Kang, YR Kim, JH Kang, JK Yoo, HY Cho, JK Kim, CH Kim, JH Park, CH Lee*.  Novel anti-nociceptive effects of cardamonin via blocking expression of cyclooxygenase-2 and transglutamisase-2. Pharmacol Biochem Behav. 118:10-15. 2014

JK Yoo, SJ Choi, JK Kim*. Expression profiles of subtracted mRNAs during cellular senescence in human mesenchymal stem cells derived from bone marrow. Exp Gerontol. 48:464-71. 2013

JH Kim, SG Park, JY Song, JK Kim, JH Sung*. Reactive oxygen species-responsive miR-210 regulates proliferation and migration of adipose-derived stem cells via PTPN2. Cell Death & Disease4, e588; doi:10.1038/cddis.2013.117. 2013

SA An, J Kim, OJ Kim, JK Kim, NK Kim, J Song, SH Oh*. Limited clinical value of multiple blood markers in the diagnosis of ischemic stroke. Clin Biochem. 46(9):710-715. 2013

JK Yoo, HY Jung, CH Kim, WS Son, JK Kim*. miR-7641 modulates the expression of CXCL1 during endothelial differentiation derived from human embryonic stem cells. Arch Pharm Res. 36:353-8. 2013

SY Park, J Kim, OJ Kim, JK Kim, J Song, DA Shin, SH Oh*. Predictive value of circulating interleukin-6 and heart-type fatty acid binding protein for 3 months clinical outcome in acute cerebral infarction: multiple blood markers profiling study. Crit Care. 2013 Mar 16;17(2):R45. 2013

JM Lee, JK Yoo, H Yoo, HY Jung, DR Lee, HC Jeong, SH Oh, HM Chung, JK Kim*. The Novel miR-7515 Decreases the Proliferation and Migration of Human Lung Cancer Cells by Targeting c-Met. Molecular Cancer Research 11:43-53. 2013

SY Jeon, HJ Lee, KH Na, DH Cha, JK Kim, JW Park, TK Yoon, GJ Kim*Hypoxia-induced downregulation of XIAP in trophoblasts mediates apoptosis via interaction with IMUP-2: Implications for placental development during pre-eclampsia. J. Cell. Biochem.114(1)89-98. 2013

SH Oh, MH Kim, JH Song, SY Park, OJ Kim, Hanna Yoo, YK Kim, JK Kim*. Alteration of immunologic responses on peripheral blood in the acute phase of ischemic stroke: Blood genomic profiling study. J Neuroimmunol. 249(1-2):60-65. 2012

JK Yoo, Jm Kim, SJ Choi, HM Noh, YD, Kwon, HN Yoo, HS Yi, HM Chung, JK Kim*. Discovery and Chracterization of Novel MicroRNAs During Endothelial Differentiation of Human Embryonic Stem Cells. Stem Cells Dev. 21(11):2049-57. 2012

JK Kim, KJ Choi, MH Lee, MH Jo, SH Kim*. Molecular imaging of a cancer-targeting theragnostics probe using a nucleolin apatamer and microRNA-221 molecular beacon-conjugated nanoparticle. Biomaterials. 33:207-217. 2012

HN Yoo, JK Yoo, JM Lee, DR Lee, JJ Ko, SH Oh, YK Choo, JK Kim*. The hsa-miR-5787 represses cellular growth by targeting eukaryotic translation initiation factor 5 (eIF5) in fibroblast. BBRC. 415: 567-572. 2011

JK Yoo, JM Kim, SJ Choi, CH Kim, DR Lee, HM Chung, JK Kim*. The hsa-miR-5739 modulates the endoglin network in endothelial cells derived from human Embryonic Stem cells. BBRC. 415:258-262. 2011

MJ Kim, KS Shin, JH Jeon, DR Lee, SH Shim, JK Kim, DH Cha, TK Yoon, GJ Kim*. Human chorionic-palte-derived mesenchymal stem cells and Wharton’s jelly-derived mesenchymal stem cells a comparartive analysis of their potential as placenta-derived stem cells. Cell Tissue Res. 346:53-64. 2011

HC Jeong, EK Kim, JH Lee, JM Lee, HN Yoo, JK Kim*. Aberrant of let-7a miRNA in the blood of non-small cell lung cancer patients. MMR. 4:383-387. 2011

HJ Lee, KE Cha, SG Hwang, JK Kim*, GJ Kim*. In vitro screening system for hepatotoxicity: comparison of bone marrow-derived mesenchymal stem cells and placenta-derived stem cells. J. Cell. Biochem. 112(1), 49-58. 2011

JS Ahn, SH Moon, JM Kim, HM Chung, JK Kim*. Identification of differentially expressed genes in human embryonic stem cell derived endothelial like cells using suppression subtractive hybridization. Stem Cells Dev. 19(8):1249-1256. 2010

Lim JJ, Sung SY, Kim HJ, Song SH, Hong JY, Yoon TK, Kim JK, Kim KS, Lee DR*. Long-term proliferation and characterization of human spermatogonial stem cells obtained from obstructive and non-obstructive azoospermia under exogenous feeder-free culture conditions. Cell Prolif. 43(4):405-417. 2010

SH Oh, NK Kim, SH Kim, JK Kim, HS Kim, WC Kim, OJ Kim*. The prevalence and risk factor analysis of silent brain infarction in patients with first-ever ischemic stroke. J Neurol Sci. 293(1-2):97-101. 2010

SY Jeon, HJ Lee, JM Park, HM Jung, JK Yoo, HJ Lee, JS Lee, DH Cha, JK Kim, GJ Kim*. Increased immortalization-upregulated protein 2 (IMUP-2) by hypoxia induces apoptosis of the trophoblast and pre-eclampsia. J. Cell. Biochem. 110(2):522-530. 2010

HM Jung, JK Kim*. Identification and characterization of WSG, a fusion gene associated with the proliferation of the WI-38 VA13 cells. Exp Lung Res. 36(3):140-147. 2010

JK Yoo, JJ Lim, JJ Ko, DR Lee*, JK Kim*. Expression profile of genes identified in human spermatogonial stem cell-like cells using suppression subtractive hybridization. J. Cell. Biochem 110(3):752-762. 2010

HM Jung, SJ Choi, JK Kim*. Expression profiles of SV40-immortalization-associated genes upregulated in various human cancers. J. Cell. Biochem. 106(4):703-713. 2009

SJ Kim, HJ An, HJ Kim, HM Jung, SY Lee, JJ Ko, IH Kim, N. Sakuragi, JK Kim*. IMUP-1 and IMUP-2 Overexpression in Endometrial Carcinoma in Korean and Japanese Populations. Anticancer Res. 8(2A):865-872. 2008

HJ An, KI Kim, JY Kim, JY Shim, HY Kang, TH Kim, JK Kim, JM Kim, SY Lee, SJ Kim*. Microsatellite instability in endometrioid type endometrial adenocarcinoma is associated with poor prognostic indicators. Am J Surg Pathol. 31(6):846-853. 2007

ZY Ryoo, BK Jung, SR Lee, MO Kim, SH Kim, HJ Kim, JY Ahn, TH Lee, YH Choi, JH Park, JK Kim*. Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts. BBRC. 349(3):995-1002. 2006

JK Kim, SH Kim, JH Han, HJ Kim, SY Chong, SP Hong, SG Hwang, JY Ahn, KY Cha, DY Oh, NK Kim*. Polymorphisms of 5, 10 – Methylenetetrahydrofolate Reductase and Risk of Stomach Cancer in a Korean Population. Anticancer Res. 25(3B):2249-2252. 2005

JY Ahn, SS Chung, BH Lee, SH Kim, PH Yoon, JY Joo, JK Kim*. Treatment of spontaneous arterial dissections with stent placement for preservation of the parent artery. Acta Neurochir. 147(3):265-73. 2005

SC Yoon, JK Kim, DH Kwak, JJ Ko, S Lee, YK Choo, WH Woo, KY Jung*. Antitumor activity of Soamsan, a traditional Korean medicine, via suppressing angiogenesis and growth factor transcription. J. Neurol. Neurosurg.  Psychiatry (JNNP) 75(6):847-851. 2004

JK Kim, KS Kim, JY Ahn, NK Kim, HM Chung, WJ Yun, KY Cha*. Enhanced apoptosis by a novel gene, Bak-like, that lacks the BH3 domain. Biochem. Biophys. Res. Commun. (BBRC) 316:18-23. 2004

JK Kim, HJ An, NK Kim, JY Ahn, KS Kim, YJ Kang, JJ Ko, DY Oh, C Lee, SJ Kim, KY Cha*. IMUP-1 and IMUP-2 Genes are Upregulated in Human Ovarian Epithelial Tumors. Anticancer Res. 23:4709-4714. 2003

JK Kim, DY Oh, SH Cho, OJ Kim, YK Cho, SH Kim, YJ Kang, JY Ahn, SG Whang, KY Cha, NK Kim*. Genetic Polymorphisms of Thymidylate Synthase Enhancer Region (TSER) in Korean. Korea J. Genetics. 25(4):287-291. 2003

NK Kim, JY Ahn, JH Song, JK Kim, JH Han, HJ An, HM Chung, JY Joo, JU Choi, KS Lee, R Roy, DY Oh*. Expression of the DNA Repair Enzyme, N-Methylpurine-DNA Glycosylase (MPG) in Astrocytic Tumors. Anticancer Res. 23:1417-1424. 2003

DH Kwak, JK Kim, JY Kim, HY Jeong, KS Keum, SH Han, YI Rho, WH Woo, KY Jung, BK Choi, YK Choo*. Anti-angiogenic activities of Cnidium officinale Makino and Tabanus bovinus. J. Ethnopharmacol 81(3):373-379. 2002

HG Choi, JK Kim, DH Kwak, JR Cho, JY Kim, BJ Kim, KY Jung, BK Choi, MK Shin. YK Choo*. Effects of high molecular weight water-soluble chitosan on in vitro fertilization and ovulation in mice fed a high-fat diet. Arch.Pharm.Res.  25(2):178-183. 2002

KY Jung, BH Kim, MR Hwang, JR Cho, HM Kim, YC Lee, CH Kim, JK Kim, BJ Kim, YK Choo*. Differential Distribution of Ganglioside GM3 in Seminiferous Tubule and Epididymis of Adult Rats. Arch.Pharm.Res. 24(4):360-366. 2001

JK Kim, R Ryll, Y Ishizuka, S Kato*. Identification of cDNAs encoding two novel nuclear proteins, IMUP-1 and IMUP-2, upregulated in SV40-immortalized human fibroblasts. Gene. 257:327-334. 2000

Y Shimada, M Nakano, N Kanda, K Murofushi, JK Kim, T Ide, H Morufushi*. Cell cycle-dependent activation of telomerase in naturally synchronized culture of a ture Slime Mold, Physarum polycephalum. Biochem Biophys Res. Commun. 232:492-496. 1997

H Tahara, K Kamada, E Sato, N Tsuyama, JK Kim, E Hara, K Oda, T Ide*. Increase in express levels of interferon-inducible genes in senescent human diploid fibroblasts and in SV40-transformed human fibroblasts with extended lifespan. Oncogene. 11:1125-1132. 1995

김진경*. 노화세포에서 발현 변화하는 신규 유전자의 cloning. 한국교육재단 학술논총(자연과학편). 2:89-92. 1994

<특허출원>

 

국외특허

사람 핵단백질을 코드하는 cDNA 국외출원(2001-218585). S.Kato, J.K.Kim, R.Ryll. 2001

 

국내특허

SV40에 의해 발현이 상승하는 신규 핵단백질인 IMUP-1 및 이를 암호화하는 cDNA. 국내등록(제0423550호) 김진경, 정형민, 차광렬. 2004

SV40에 의해 발현이 상승하는 신규 핵단백질인 IMUP-2 및 이를 암호화하는 cDNA. 국내등록(제0423549호) 김진경, 정형민, 차광렬. 2004

인간 중배엽줄기세포 유래의 신규 miRNA. 국내등록 (제10-1133559호) 김진경, 정형민, 노혜민, 유중기. 2009

신규한 miRNA hc-smR-S2-5 및 이의 용도. 국내등록 (제10-1308924호) 김진경, 정형민, 유중기, 이지민. 2013

신규한 miRNA hc-smR-S2-3 및 이의 용도. 국내출원 (10-2013-0089718) 김진경, 유중기, 이지민, 정호용. 2013