[연구내용]
생명체는 다양한 기능을 수행하는 세포라는 단위로 구성되어 있으며, 이들은 유전자들에 저장된 정보의 발현 산물인 단백질들의 조합으로 이루어져 있다. 생명현상의 유지를 위해 세포들간의 정보교환이 이루어져야 하는데 이들은 신호전달 (Signal Transduction)이라는 과정을 통해 형성된다. 이러한 일련의 신호전달과정은 단백질 분자간의 네트웍이 조절됨으로서 가능하며 이 과정의 이상은 암, 당뇨, 치매, 불임등과 같은 질병들의 직접 또는 간접적인 원인이 된다. 따라서 유전자 수준에서의 연구뿐만 아니라 단백질의 변형이나 단백질 결합 복합체의 변화에 대한 연구방법이 요구된다.
우리 분자신호전달 연구실에서는 암세포, 역분화/줄기세포 (인간과 마우스) 및 임파구 신호전달체계에서 단백질분해과정 및 세포의 성장과 분화를 조절할 것으로 생각되는 단백질분해조절 효소 (Deubiquitinating enzyme: 탈유비퀴틴화 효소)의 생물학적 기전을 규명하고 있다. 최근 이들 세포에서 새로운 탈유비퀴틴화 효소 유전자들을 발굴했으며, 이들의 생물학적 기능을 이해하기 위해 분자유전학 기법을 이용하여 연구하고 있다. 이 프로젝트의 목표는 암세포, 줄기세포, 및 임파구의 성장과 분화를 조절할 수 있는 새로운 방법을 찾는 것이다.
두 번째 연구영역으로는 습관성 유산 및 다낭성 난소증후군을 유발하는 유전자 및 유발요소를 찾고 분자적 기전과 역할을 규명함으로써 궁극적으로는 세포 및 유전자치료를 이용하는 임상적 응용에 연구의 초점을 맞추고 있다. 특히 임신의 과정은 빠른 유전자 발현 양상의 변화가 요구하므로 신호전달계에서의 조절이 잘못 될 경우 바로 태아성장의 실패와 연결된다는 점에서 그 중요성이 더욱 강조되고 있다. 본 연구실에서는 습관성 유산질환 및 다낭성 난소증후군 관련 유전체 및 단백체를 찾기위해 정상과 질환 환자로부터 얻은 샘플로부터 cDNA subtraction method, DD-PCR, 및 2DE-PAGE/MALDI-TOF/MS 분석등을 이용하여 정상인과 환자와의 유전자 발현의 차이를 비교함으로서 습관성 유산질환 및 다낭성 난소증후군의 진단과 치료의 방법을 개발하고자 한다.
[연구논문] (총 국외 SCI 논문 158편 중 최근 5년간: 2019-현재) (*: Corresponding author)
- 161. Park, H.-B., and Baek, K.-H. (2023). Current and future directions of USP7 interactome in cancer study. BBA-Rev. on Cancer, 1878: 188992. (Corresponding author)
- 160. Park, S.-S., and Baek, K.-H. (2023). Synergistic effect of YOD1 and USP21 on the Hippo signaling pathway. Cancer Cell Int. 23: 209. (Corresponding author)
- 159. Pei, C.-Z., Seok, J., Kim, K.J., Choi, B.-C., and Baek, K.-H. (2023). Deficiency of HtrA4 in BeWo cells downregulates angiogenesis through IL-6/JAK/STAT3 signaling. Biomed. Pharmacother. 166: 115288. (Corresponding author)
- 158. Pei, C.-Z. Choi, B.-C., Park, J.-H., Park, H. Y., Paek, J., Lee, K.-J., Yun, B.-S., Kim, Y. J., and Baek, K.-H. (2023). Cellular functions of high-temperature requirement factor A4 in placenta. Cells, 12: 1459. (Corresponding author)
- 157. Kang, S.-J., Pei, C.-Z., Lee, D.-H., Ha, J.-E., and Baek, K.-H. (2023). A pilot randomized clinical trial of biomedical link with mental health in art therapy intervention programs for alcohol use disorder: Changes in NK cells, addiction biomarkers, electroencephalography, and MMPI-2 profiles. PLoS One, 18 (5): e0284344. (Corresponding author)
- 156. Park, H.-B., Min, Y., Hwang, S., and Baek, K.-H. (2023). Suppression of USP7 negatively regulates the stability of ETS proto-oncogene 2 protein. Biomed. Pharmacother. 162: 114700. (Corresponding author)
- 155. Min, Y., Park, H.-B., Baek, K.-H., and Hwang, S. (2023). Cellular functions of deubiquitinating enzymes in ovarian adenocarcinoma. Genes, 14: 886. (Co-corresponding author)
- 154. Park, H.-B., Choi, B.-C., and Baek, K.-H. (2023). PGK1 modulates balance between pro- and anti-inflammatory cytokines by interacting with ITI-H4. Biomed. Pharmacother. 161: 114437. (Corresponding author)
- 153. Park, S.-S., Park; Do, H.-A., Park, H.-B., Choi, H.-S., and Baek, K.-H. (2023). Deubiquitinating enzyme YOD1 deubiquitinates and destabilizes α-synuclein. Biochem. Biophys. Res. Commun. 645: 124-131. (Corresponding author)
- 152. Lee, D.-G., Kim, Y.-K., and Baek, K.-H. (2022). The bHLH transcription factors in neural development and therapeutic applications for neurodegenerative diseases. Int. J. Mol. Sci. 23: 13936. (Corresponding author)
- 151. Choi, H.-S., Lim, E.-S., and Baek, K.-H. (2022). Deubiquitinating enzyme USP12 regulates the pro-apoptosis protein Bax. Int. J. Mol. Sci. 23: 13107. (Corresponding author)
- 150. Kim, S.-H., and Baek, K.-H. (2022). Ovarian tumor deubiquitinase 6A regulates cell proliferation via deubiquitination of nucleolin and caspase-7. Int. J. Oncol. in press.
- 149. Park, H.-B, Hwang, S., and Baek, K.-H. (2022). USP7 regulates the ERK1/2 signaling pathway through deubiquitinating Raf-1 in lung adenocarcinoma. Cell Death & Dis. in press.
- 148. Kim, S. Y., Lee, J.-Y., Cho, Y.-J., Jo, K. H., Kim, E. S., Han, J. H., Baek, K.-H., and Moon, S. D. (2022). USP37 deubiquitinates CDC73 in HPT-JT syndrome. Int. J. Mol. Sci. 23: 6364.
- 147. Park, H.-B., and Baek, K.-H. (2022). E3 ligases and deubiquitinating enzymes regulating the MAPK signaling pathway in cancers. BBA – Rev. on Cancer. 1877: 188736.
- 146. Do, H.-A., and Baek, K.-H. (2022). Protein phosphatase 2A regulated by USP7 is polyubiquitinated and polyneddylated. Oncol. Rep. 48: 124.
- 145. Choi, H.-S., and Baek, K.-H.(2022). Pro-apoptotic and anti-apoptotic regulation mediated by deubiquitinating enzymes. Cell. Mol. Life Sci. 79: 117.
- 144. Park, S.-S., and Baek, K.-H.(2022). Acute myeloid leukemia-related proteins modified by ubiquitin and ubiquitin-like proteins. Int. J. Mol. Sci. 23: 514.
- 143. Pei, C.-Z., Jin, L., and Baek, K.-H.(2021). Pathogenetic analysis of polycystic ovary syndrome from the perspective of omics. Biomed. Pharmacother. 142: 112031.
- 142. Kim, M.-S., Kim, K., Oh, S.-K., Lee, G., Kim, J.-O., Li, L., Park, J.-H., and Baek, K.-H. (2021). Long-lasting growth hormone regulated by the ubiquitin-proteasome system. Int. J. Mol. Sci. 22: 6268.
- 141. Kim, S.-H., and Baek, K.-H.(2021). Regulation of cancer metabolism by deubiquitinating enzymes: the Warburg effect. Int. J. Mol. Sci. 22: 6173.
- 140. Kang, S.-J., Kim, H.-S., and Baek, K.-H.(2021). Effects of nature-based group art therapy programs on stress, self-esteem, and changes in electroencephalogram (EEG) in non-disabled siblings of children with disabilities. Int. J. Environ. Res. Public Health, 18: 5912.
- 139. Do, H.-A., and Baek, K.-H.(2021). Cellular functions regulated by deubiquitinating enzymes in neurodegenerative diseases. Ageing Res. Rev. 69: 101367.
- 138. Hur, J. Yoo, J., Shin, D., Baek, K.-H., Park, S., and Lee, K.J. (2021). Predictability of macrosomic birth based on fetal factors and fetal aneuploidy screening biochemical markers in hyperglycemic mothers. Int. J. Med. Sci. 18(12): 2653-2660.
- 137. Park, J., Park, Y., Koh, I., Kim, N. K., Baek, K.-H., Yun, B.-S., Lee, K.-J., Song, J. Y., Lee, E., and Kwack, K. (2020). Association of an APBA3 missense variant with risk of premature ovarian failure in the Korean female population. J. Pers. Med. 10(4): E193.
- 136. Shin, J.-W., Lee, J.-h., Kim, H., Lee, D.-H., Baek, K.-H., Sunwoo, J.-S., Byun, J.-I., Kim, T.-J., Jun, J.-S., Han, D., and Jung, K.-Y. (2020). Bioinformatic analysis of proteomic data for iron, inflammation, and hypoxic pathways in restless legs syndrome. Sleep Med. 75: 448-455.
- 135. Choi, H.-S., Pei, C.-Z., Park, J.-H., Kim, S.-Y., Song, S.-Y., Shin, G.-J., and Baek, K.-H. (2020). Protein stability of pyruvate kinase isozyme M2 is mediated by HAUSP. Cancers, 12: 1548.
- 134. Lim, K.-H., Joo, J.-Y., and Baek, K.-H., (2020). The potential roles of deubiquitinating enzymes in brain diseases. Ageing Res. Rev. 61: 101088.
- 133. Park, H.-B., Kim, J.-W., and Baek, K.-H. (2020). Regulation of Wnt signaling through ubiquitination and deubiquitination in cancers. Int. J. Mol. Sci. 21(11): E3904.
- 132. Baek, K.-H., Choi, J., and Pei, C.-Z. (2020). Cellular functions of OCT-3/4 regulated by ubiquitination in proliferating cells. Cancers, 12(3): 663.
- 131. Lee, D.-H., Park, J.-H., Choi, J., Lee, K.-J., Yun, B.-S., and Baek, K.-H.(2020). Differential expression of DUB genes in ovarian cells treated with di-2-ethylhexyl phthalate. Int. J. Mol. Sci. 21(5): 1755.
- 130. Park, J.-H., Kim, S.-Y., Cho, H.-J., Lee, S.-Y., and Baek, K.-H.(2020). YOD1 deubiquitinates NEDD4 involved in the Hippo signaling pathway. Cell. Physiol. Biochem. 54: 1-14.
- 129. Woo, B., and Baek, K.-H.(2019). Interplay of deubiquitinating enzymes and cytokines. Cyt. Growth Factor Rev. 48: 40-51.
- 128. Lee, H.-R., and Baek, K.-H.(2019). The role of natural killer cells for immunotherapy in chronic myeloid leukemia (CML). Oncol. Rep. 41(5): 2625-2635.
- 127. Kim, S.-Y. and Baek, K.-H.(2019). TGF-β signaling pathway mediated by deubiquitinating enzymes. Cell. Mol. Life Sci. 76(4): 653-665.
- 126. Lee, D.-H., Pei, C.-Z., Song, J.-Y., Lee, K.-J., Yun, B.-S., Kwack, K.-B., Lee, E.-I., and Baek, K.-H.(2019). Identification of serum biomarkers for premature ovarian failure. BBA – Proteins and Proteomics, 1867(3): 219-226.
논문 (국내/외 비SCI 논문 18편 중 최근 5년간: 2017-현재) (*: Corresponding author)
- 18. Pei, C.-Z, Kim, Y. J., and Baek, K.-H. (2019). Pathogenetic factors involved in recurrent pregnancy loss from multiple aspects. Obstetrics & Gynecology Science, 62(4): 212-223.
- 17. Kang, S.-J. Kim, S.-H., and Baek, K.-H. (2018). Effects of short-term clinical art therapy in firefighters with post-traumatic stress risk. J Complement Med & Alt Healthcare, 8(4): 001-008.
- 16. Baek, K.-H. (2018). Regulation of protein degradation and homeostasis by the cytokine-inducible deubiquitinating enzymes. Int. J. Immunother Cancer Res. 4(1): 1-3.